Bacterial growth on surfaces: automated image analysis for quantification of growth rate-related parameters
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چکیده
منابع مشابه
Bacterial growth on surfaces: automated image analysis for quantification of growth rate-related parameters.
A fast routine method for estimating bacterial cell growth rates by using the metachromatic dye acridine orange is described. The method allows simultaneous estimates of cellular RNA and DNA contents of single cells. Acridine orange staining can be used as a nonspecific supplement to quantitative species-specific hybridizations with fluorescence-labelled ribosomal probes to estimate the single-...
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The state-of-the-art technology using flow-chamber microscopy imaging enables us to gain insight into the arcana of bacterial cell growth. However, a large number of high resolution developmental image data sets are produced that need to be properly processed and analyzed. The mathematical challenge lies in the automated image analysis, extraction of cell size profiles and determination of the ...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1995
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.61.2.741-748.1995